Specific detection of Brucella DNA by PCR.

  title={Specific detection of Brucella DNA by PCR.},
  author={Carolina Romero and Carlos Gamazo and Margarita Pardo and Ignacio L{\'o}pez-Go{\~n}i},
  journal={Journal of clinical microbiology},
  volume={33 3},
A PCR assay with primers derived from the 16S rRNA sequence of Brucella abortus was developed. Nine different combinations between six primers were tested. One pair of primers, which amplified a 905-bp fragment, was selected. As little as 80 fg of Brucella DNA was detected by this method. DNAs from all of the representative strains of the species and biovars of Brucella and from 23 different Brucella isolates were analyzed and yielded exclusively the 905-bp fragment. No amplification was… CONTINUE READING

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