Specific detection of Arcobacter and Campylobacter strains in water and sewage by PCR and fluorescent in situ hybridization.

@article{Moreno2003SpecificDO,
  title={Specific detection of Arcobacter and Campylobacter strains in water and sewage by PCR and fluorescent in situ hybridization.},
  author={Yolanda Moreno and Salut Botella and Jos{\'e} Luis Alonso and Mar{\'i}a Antonia Ferr{\'u}s and Manuel Hern{\'a}ndez and J. Vergara Hern{\'a}ndez},
  journal={Applied and environmental microbiology},
  year={2003},
  volume={69 2},
  pages={1181-6}
}
The aim of this study was to evaluate PCR and fluorescent in situ hybridization (FISH) techniques for detecting Arcobacter and Campylobacter strains in river water and wastewater samples. Both 16S and 23S rRNA sequence data were used to design specific primers and oligonucleotide probes for PCR and FISH analyses, respectively. In order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells… CONTINUE READING