Specific detection and typing of Mycoplasma synoviae strains in poultry with PCR and DNA sequence analysis targeting the hemagglutinin encoding gene vlhA.

@article{Hong2004SpecificDA,
  title={Specific detection and typing of Mycoplasma synoviae strains in poultry with PCR and DNA sequence analysis targeting the hemagglutinin encoding gene vlhA.},
  author={Yang Hong and M. I. Zamora Garc{\'i}a and Victoria Leiting and Dulan Bencina and Louise Dufour-Zavala and Guillermo Zavala and Stanley H. Kleven},
  journal={Avian diseases},
  year={2004},
  volume={48 3},
  pages={606-16}
}
Mycoplasma synoviae is a major pathogen of chickens and turkeys, causing economic losses to the poultry industry worldwide. In this study, we validated and applied polymerase chain reaction (PCR) and DNA sequence analysis on the N-terminal end of the hemagglutinin encoding gene vlhA as an alternative for the detection and initial typing of field strains of M. synoviae in commercial poultry. PCR primers were tested against isolates of M. synoviae from various sources along with other avian… CONTINUE READING

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