Specific dephosphorylation of phosphopeptides by the yeast alkaline phosphatase encoded by PHO8 gene.

@article{DonellaDeana1993SpecificDO,
  title={Specific dephosphorylation of phosphopeptides by the yeast alkaline phosphatase encoded by PHO8 gene.},
  author={Arianna Donella-Deana and Sa{\vs}a Ostoji{\'c} and Lorenzo A Pinna and Slobodan Barbaric},
  journal={Biochimica et biophysica acta},
  year={1993},
  volume={1177 2},
  pages={221-8}
}
Partially purified nonspecific phosphate-repressible alkaline phosphatase from Saccharomyces cerevisiae encoded by PHO8 gene (rALPase), efficiently dephosphorylates phosphohistones and a variety of phosphopeptides. The pho8 mutant, constructed by disruption of the chromosomal counterpart of the PHO8 gene, is lacking in phosphatase activity toward phosphopeptides, confirming that this activity is actually due to rALPase. rALPase activity tested on phosphopeptides is maximum in the pH range 6.5-7… CONTINUE READING