Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosomes using fluorescent in situ hybridization (FISH)

@article{Piumi2000SpecificCL,
  title={Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosomes using fluorescent in situ hybridization (FISH)},
  author={François Piumi and Daniel Vaiman and Edmond Paul Cribiu and Bernard Gu{\'e}rin and Patrice Humblot},
  journal={Genetics, Selection, Evolution : GSE},
  year={2000},
  volume={33},
  pages={89 - 98}
}
X and Y specific probes were identified in order to apply the fluorescent in situ hybridization (FISH) technique to bovine spermatozoa. For Y chromosome detection, the BRY4a repetitive probe, covering three quarters of the chromosome, was used. For X chromosome detection, a goat Bacterial Artificial Chromosome (BAC) specific to the X chromosome of bovine and goats and giving a strong FISH signal was used. Each probe labeled roughly 45% of sperm cells. The hybridization method will be useful for… CONTINUE READING
BETA

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