Specific covalent labeling of recombinant protein molecules inside live cells.

  title={Specific covalent labeling of recombinant protein molecules inside live cells.},
  author={B A Griffin and Stephen R. Adams and Roger Tsien},
  volume={281 5374},
Recombinant proteins containing four cysteines at the i, i + 1, i + 4, and i + 5 positions of an alpha helix were fluorescently labeled in living cells by extracellular administration of 4',5'-bis(1,3, 2-dithioarsolan-2-yl)fluorescein. This designed small ligand is membrane-permeant and nonfluorescent until it binds with high affinity and specificity to the tetracysteine domain. Such in situ labeling adds much less mass than does green fluorescent protein and offers greater versatility in… 

A general method for the covalent labeling of fusion proteins with small molecules in vivo

A general method for the covalent labeling of fusion proteins in vivo that complements existing methods for noncovalentlabeling of proteins and that may open up new ways of studying proteins in living cells is described.

Live-cell targeting of his-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes.

This work reports on the site-specific, rapid, and efficient labeling of endogenous and recombinant histidine-tagged proteins in distinct subcellular compartments using cell-penetrating multivalent chelator carrier complexes.

Affinity Conjugation for Rapid and Covalent Labeling of Proteins in Live Cells.

A method for affinity conjugation of protein with a fluorogenic probe and a "tagging-then-labeling" approach by a combination of affinity Conjugation with bioorthogonal reactions is described.

Multivalent Chelators for In Vivo Protein Labeling.

Recent applications of multivalent chelator heads, recognizing oligohistidine-tagged proteins are reported on, finding that the striking features of this system has facilitated the analysis of protein complexes by single-molecule approaches.

An engineered protein tag for multiprotein labeling in living cells.

Super-Chelators for Advanced Protein Labeling in Living Cells.

A new class of hexavalent N-nitrilotriacetic acid (hexaNTA) chelators, displaying the highest affinity and stability of all NTA-based small interaction pairs described so far are defined.

Fluorescent labeling of membrane proteins on the surface of living cells by a self-catalytic glutathione S-transferase omega 1 tag.

Imaging a specific protein of interest in live cell has versatile applications in biological research. Recently, diverse chemical tags have been developed to overcome the limits of autofluorescence

Live cell labeling of native intracellular bacterial receptors using aniline-catalyzed oxime ligation.

The first example of live cell labeling and imaging of an intracellular bacterial receptor involved in cell-to-cell communication, using a novel two-step approach involving covalent attachment of a reactive mimic of the primary endogenous Pseudomonas aeruginosa quorum-sensing signal to its receptor, LasR, followed by aniline-catalyzed oxime formation between the modified receptor and a fluorescent BODIPY derivative.

Selective chemical labeling of proteins in living cells.




Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter.

The robust change in emission ratio reveals quantitative heterogeneity in real-time gene expression, enables clonal selection by flow cytometry, and forms a basis for high-throughput screening of pharmaceutical candidate drugs in living mammalian cells.

Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin

New fluorescent indicators for Ca2+ that are genetically encoded without cofactors and are targetable to specific intracellular locations are constructed and dubbed ‘cameleons’.

The green fluorescent protein.

  • R. Tsien
  • Biology
    Annual review of biochemistry
  • 1998
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in

Crystal Structure of the Aequorea victoria Green Fluorescent Protein

The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria has generated intense interest as a marker for gene expression and localization of gene products. The

A model peptide with enhanced helicity.

Increasing the number of EAAAR sequence units in the peptide acetylW(EAAAR)nAamide from three to five indicates that the spectral features anticipated for a completely helical peptide are closely approached.

Anorganische Ringsysteme mit Ferrocenyl‐Substituenten

Ausgehend von FcPCl2 (1), FcPH2 (2), FcAsCl2 (3) und Fe(C5H4AsCl2)2 (4) werden drei-, vier-, funf- und achtgliedrige Heterocyclen mit Ferrocenyl-Substitutenten synthetisiert (Fc = Ferrocenyl). Durch