Specific cleavage of dynein heavy chains by ultraviolet irradiation in the presence of ATP and vanadate.

@article{LeeEiford1986SpecificCO,
  title={Specific cleavage of dynein heavy chains by ultraviolet irradiation in the presence of ATP and vanadate.},
  author={A Lee-Eiford and Rosaleen Ow and Ian R. Gibbons},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 5},
  pages={2337-42}
}
Irradiation of soluble dynein 1 from sea urchin sperm flagella at 254 nm in the presence of 50 microM ATP and 100 microM inorganic vanadate (Vi) cleaves the alpha and beta heavy chains into approximately equal quantities of two polypeptides of Mr 228,000 and 200,000, with a conversion efficiency of about 63%. A similar cleavage occurs in the presence of Vi and either ADP or 8-azidoadenosine 5'-triphosphate (8-N3ATP); in the latter case, 8-N3ATP becomes covalently bound principally to the Mr 228… CONTINUE READING