Specific binding of phorbol ester tumor promoters.

  title={Specific binding of phorbol ester tumor promoters.},
  author={P E Driedger and Peter M. Blumberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={77 1},
[20-(3)H]Phorbol 12,13-dibutyrate bound to particulate preparations from chicken embryo fibroblasts in a specific, saturable, reversible fashion. Equilibrium binding occurred with a K(d) of 25 nM; this value is very close to the 50% effective dose (ED(50)), 50 nM, previously determined for the biological response (induction of fibronectin loss) in growing chicken embryo fibroblasts. At saturation, 1.4 pmol of [20-(3)H]phorbol 12,13-dibutyrate was bound per mg of protein (approximately 7 x 10(4… CONTINUE READING
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