Specific benzodiazepine receptors in rat brain characterized by high-affinity (3H)diazepam binding.

  title={Specific benzodiazepine receptors in rat brain characterized by high-affinity (3H)diazepam binding.},
  author={Claus Braestrup and Richard Felt Squires},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={74 9},
  • C. Braestrup, R. Squires
  • Published 1 September 1977
  • Biology, Chemistry
  • Proceedings of the National Academy of Sciences of the United States of America
[3H]Diazepam appears to bind specifically to a single, saturable, binding site located on rat brain membranes, with an affinity constant near 3 nM at pH 7.4. Specific binding constitutes more than 90% of total binding at 0 degrees and less than 10% of total binding at 37 degrees. Arrhenius plots suggest a sharp conformational change in the diazepam receptor near 18 degrees. Mitochondrial fractions from rat kidney, liver, and lung exhibit some [3H]diazepam binding that can be displaced by… 

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