Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins.

@article{Hemelaar2004SpecificAC,
  title={Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins.},
  author={Joris Hemelaar and Anna Borodovsky and Benedikt M. Kessler and David Reverter and Julie L. Cook and Nagamallesawari Kolli and Tudev Gan-Erdene and Keith D. Wilkinson and Grace B Gill and Christopher D Lima and Hidde L. Ploegh and Huib Ovaa},
  journal={Molecular and cellular biology},
  year={2004},
  volume={24 1},
  pages={84-95}
}
Modification of proteins by ubiquitin (Ub)-like proteins (UBLs) plays an important role in many cellular processes, including cell cycle progression, nuclear transport, and autophagy. Protein modification occurs via UBL-conjugating and -deconjugating enzymes, which presumably exert a regulatory function by determining the conjugation status of the substrate proteins. To target and identify UBL-modifying enzymes, we produced Nedd8, ISG15, and SUMO-1 in Escherichia coli and equipped them with a C… CONTINUE READING