Specific amplification of rearranged immunoglobulin variable region genes from mouse hybridoma cells.

@article{GavilondoCowley1990SpecificAO,
  title={Specific amplification of rearranged immunoglobulin variable region genes from mouse hybridoma cells.},
  author={J V Gavilondo-Cowley and Maria Josefina Coloma and Jes{\'u}s V{\'a}zquez and Marta Mar{\'i} Ayala and Alfonso Guti{\'e}rrez Mac{\'i}as and Kirk E. Fry and James William Larrick},
  journal={Hybridoma},
  year={1990},
  volume={9 5},
  pages={
          407-17
        }
}
In this article we show how the polymerase chain reaction (PCR) and primers designed for conserved sequences of leader (L), framework one (FR1) and constant (CONST) regions of immunoglobulin light and heavy chain genes can be used for the cloning and sequencing of rearranged antibody variable regions from mouse hybridoma cells. RNA was extracted from the mouse hybridoma cells secreting MAbs: IOR-T3a (anti-CD3), C6 (anti-P1 of N. meningitidis B385), IOR-T1 (anti-CD6), CB-CEA.1 (anti… CONTINUE READING
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