Specific adsorption of histidine-tagged proteins on silica surfaces modified with Ni2+/NTA-derivatized poly(ethylene glycol).

@article{Kang2007SpecificAO,
  title={Specific adsorption of histidine-tagged proteins on silica surfaces modified with Ni2+/NTA-derivatized poly(ethylene glycol).},
  author={Eunah Kang and Jin-Won Park and Scott J McClellan and Jong-Mok Kim and David P Holland and Gil U. Lee and Elias I. Franses and K. -P. Park and David H. Thompson},
  journal={Langmuir : the ACS journal of surfaces and colloids},
  year={2007},
  volume={23 11},
  pages={6281-8}
}
Silica surfaces modified with nitrilotriacetic acid (NTA)-polyethylene glycol (PEG) derivatives were used to immobilize hexahistidine-tagged green fluorescent protein (His6-GFP), biotin/streptavidin-AlexaFluor555 (His6-biotin/SA-AF), and gramicidin A-containing vesicles (His6-gA). Three types of surface-reactive PEG derivatives-NTA-PEG3400-Si(OMe)3, NTA… CONTINUE READING