Specific accumulation of GFP in a non-acidic vacuolar compartment via a C-terminal propeptide-mediated sorting pathway.

@article{Sansebastiano1998SpecificAO,
  title={Specific accumulation of GFP in a non-acidic vacuolar compartment via a C-terminal propeptide-mediated sorting pathway.},
  author={Gian Pietro Di Sansebastiano and Nadine Paris and Sophie Marc-Martin and J M Neuhaus},
  journal={The Plant journal : for cell and molecular biology},
  year={1998},
  volume={15 4},
  pages={449-57}
}
The green fluorescent protein (GFP) from Aequorea victoria can be detected in living plant cells after transient transformation of protoplasts. Expression of the GFP can be used to monitor protein trafficking in a mixed cell population and also to study the different function and importance of organelles in different cell types. We developed a vacuolar form of GFP that was obtained by replacing the C-terminal endoplasmic reticulum (ER)-retention motif of mGFP5-ER by the vacuolar targeting… CONTINUE READING