Specific Gene bciD for C7-Methyl Oxidation in Bacteriochlorophyll e Biosynthesis of Brown-Colored Green Sulfur Bacteria

@article{Harada2013SpecificGB,
  title={Specific Gene bciD for C7-Methyl Oxidation in Bacteriochlorophyll e Biosynthesis of Brown-Colored Green Sulfur Bacteria},
  author={J. Harada and T. Mizoguchi and S. Satoh and Y. Tsukatani and M. Yokono and M. Noguchi and A. Tanaka and H. Tamiaki},
  journal={PLoS ONE},
  year={2013},
  volume={8}
}
The gene named bciD, which encodes the enzyme involved in C7-formylation in bacteriochlorophyll e biosynthesis, was found and investigated by insertional inactivation in the brown-colored green sulfur bacterium Chlorobaculum limnaeum (previously called Chlorobium phaeobacteroides). The bciD mutant cells were green in color, and accumulated bacteriochlorophyll c homologs bearing the 7-methyl group, compared to C7-formylated BChl e homologs in the wild type. BChl-c homolog compositions in the… Expand
Inactivation of bciD and bchU genes in the green sulfur bacterium Chlorobaculum limnaeum and alteration of photosynthetic pigments in the resultant mutants
Abstract The bciD and bchU genes were earlier identified as relating to the C7-formylation and to catalyzing the C20-methylation, respectively, of bacteriochlorophyll(BChl)- e biosynthesis in theExpand
Isolation and structural determination of C8-vinyl-bacteriochlorophyll d from the bciA and bchU double mutant of the green sulfur bacterium Chlorobaculum tepidum
AbstractThe mutant lacking enzymes BciA and BchU, that catalyzed reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfurExpand
Over-expression of the C82-methyltransferase BchQ in mutant strains of the green sulfur bacterium Chlorobaculum limnaeum for synthesis of C8-hyper-alkylated chlorosomal pigments
Abstract The pigment 8-isobutyl-12-ethyl-([,][I,E])bacteriochlorophyll (BChl) c is key to chlorosomal self-aggregation structures in vivo and was found to produce an organogel in carbon tetrachlorideExpand
Isolation and characterization of a new bacteriochlorophyll-c bearing a neopentyl substituent at the 8-position from the bciD-deletion mutant of the brown-colored green sulfur bacterium Chlorobaculum limnaeum
TLDR
The mutant of the brown-colored green sulfur bacterium Chlorobaculum limnaeum lacking BciD which was responsible for formation of a formyl group at the 7-position in bacteriochlorophyll(BChl)-e biosynthesis was constructed and a new BChl-c bearing the bulkiest, triple 82-methylated neopentyl substituent at the 8-position was identified. Expand
A paralog of a bacteriochlorophyll biosynthesis enzyme catalyzes the formation of 1,2-dihydrocarotenoids in green sulfur bacteria
TLDR
It is demonstrated that a bchO mutant is unable to synthesize 1′,2′-dihydrochlorobactene, and accordingly and consistent with its role in carotenoid biosynthesis, it is proposed to rename the gene cruI. Expand
In Vivo Energy Transfer from Bacteriochlorophyll c, d, e, or f to Bacteriochlorophyll a in Wild‐Type and Mutant Cells of the Green Sulfur Bacterium Chlorobaculum limnaeum
TLDR
The energy-transfer from the chlorosomal aggregates to BChl-a in these mutant cells was observed at 77 K by using the picosecond time-resolved fluorescence measurements and according to the Forster energy transfer mechanism, the energy- transfer efficiency was higher in the order of BChls c, d, e, and f. Expand
In Vitro Assays of BciC Showing C132-Demethoxycarbonylase Activity Requisite for Biosynthesis of Chlorosomal Chlorophyll Pigments.
TLDR
The BciC derived from the green sulfur bacterium Chlorobaculum tepidum was characterized and the in vitro enzymatic activities of its recombinant protein were examined, and this enzyme was effective for diverting the chlorosomal pigment biosynthetic pathway at the stage of Chlide a away from syntheses of other pigments. Expand
BciD Is a Radical S-Adenosyl-l-methionine (SAM) Enzyme That Completes Bacteriochlorophyllide e Biosynthesis by Oxidizing a Methyl Group into a Formyl Group at C-7*
Green bacteria are chlorophotorophs that synthesize bacteriochlorophyll (BChl) c, d, or e, which assemble into supramolecular, nanotubular structures in large light-harvesting structures calledExpand
Light-dependent accumulation of new bacteriochlorophyll-e bearing a vinyl group at the 8-position in the green sulfur bacterium Chlorobaculum limnaeum
Abstract The green sulfur bacterium Chlorobaculum limnaeum producing bacteriochlorophyll(BChl)-e was anaerobically cultured by illumination using red LED light at around 660 nm. When the bacteriumExpand
Biosynthesis of chlorophylls and bacteriochlorophylls in green bacteria
Abstract Green bacteria include chlorophototrophic members of the phyla Chlorobi, Chloroflexi, and Acidobacteria and are defined by their use of chlorosomes for light-harvesting. Despite their sharedExpand
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References

SHOWING 1-10 OF 39 REFERENCES
Bacteriochlorophyllide c C-82 and C-121 Methyltransferases Are Essential for Adaptation to Low Light in Chlorobaculum tepidum
TLDR
Observations show that the C-8(2) and C-12(1) methylations of BChl c play important roles in the adaptation of C. tepidum to low light intensity and suggest that these methylations also directly or indirectly affect the regulation of the BChL c biosynthetic pathway. Expand
Chlorobium tepidum Mutant Lacking Bacteriochlorophyll c Made by Inactivation of the bchK Gene, Encoding Bacteriochlorophyll c Synthase
TLDR
The gene encoding bacteriochlorophyll (BChl) c synthase was identified by insertional inactivation in the photosynthetic green sulfur bacterium Chlorobium tepidum and was named bchK, which suggests that the Photosynthetic ancestor of green sulfur bacteria could have evolved without chlorosomes and BChl c and instead used only BChL a-containing proteins as the major light-harvesting antennae. Expand
Identification of a Gene Essential for the First Committed Step in the Biosynthesis of Bacteriochlorophyll c*
TLDR
The potential mechanism by which BciC removes the C-132-methylcarboxyl moiety of chlorophyllide a is discussed and its roles in the first committed step of BChl c, d, and e biosynthesis are indicated. Expand
Chlorobium Tepidum: Insights into the Structure, Physiology, and Metabolism of a Green Sulfur Bacterium Derived from the Complete Genome Sequence
TLDR
Surprisingly, some structural components and biosynthetic pathways associated with photosynthesis and energy metabolism in Chl. Expand
Bacteriochlorophyll f: properties of chlorosomes containing the “forbidden chlorophyll”
TLDR
Fluorescence spectroscopy showed that energy transfer to the baseplate was much less efficient in chlorosomes containing BChl f than in WT chlorosome containing Bchl e, which may explain why BChL f is not observed naturally. Expand
Isorenieratene Biosynthesis in Green Sulfur Bacteria Requires the Cooperative Actions of Two Carotenoid Cyclases
ABSTRACT The cyclization of lycopene to γ- or β-carotene is a major branch point in the biosynthesis of carotenoids in photosynthetic bacteria. Four families of carotenoid cyclases are known, andExpand
Characterization of Chlorophyll a and Bacteriochlorophyll a Synthases by Heterologous Expression in Escherichia coli*
TLDR
In vitro enzymatic assays indicated that heterologous expression of the chlG and bchG gene products in Escherichia coli conferred chlorophyll a and bacteriochlorophyllA synthase activity, respectively. Expand
In vitro synthesis and characterization of bacteriochlorophyll-f and its absence in bacteriochlorophyll-e producing organisms
TLDR
Bacteriochlorophyll(BChl)-f was below the level of detection in three strains of green photosynthetic bacteria producing BChl-e, and compared with other naturally occurring chlorophyll pigments obtained from the main light-harvesting antenna systems of green sulfur bacteria, BChls-c/d/e. Expand
Accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence in the CT2256-deleted mutant of the green sulfur bacterium Chlorobium tepidum.
  • J. Harada, Shohei Miyago, +4 authors H. Oh-oka
  • Biology, Medicine
  • Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
  • 2008
TLDR
The deletion mutants of the CT1232 and CT2256 genes were constructed using an insertional inactivation method in order to clarify the biosynthetic process of the Delta2,6-phytadienyl and phytyl groups in green sulfur bacteria. Expand
Chlorophyll a oxygenase (CAO) is involved in chlorophyll b formation from chlorophyll a.
TLDR
It is demonstrated that a chlorophyll a oxygenase is involved inchlorophyll b formation and that an overlapping region of a nuclear genome was deleted in all mutants and that this encodes a protein whose sequence is similar to those of methyl monooxygenases. Expand
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