Specific Ablation of the Apoptotic Functions of Cytochrome c Reveals a Differential Requirement for Cytochrome c and Apaf-1 in Apoptosis

  title={Specific Ablation of the Apoptotic Functions of Cytochrome c Reveals a Differential Requirement for Cytochrome c and Apaf-1 in Apoptosis},
  author={Z. Hao and G. Duncan and Chia-Che Chang and A. Elia and M. Fang and A. Wakeham and H. Okada and T. Calzascia and YingJu Jang and A. You-Ten and W. Yeh and P. Ohashi and X. Wang and T. Mak},
  • Z. Hao, G. Duncan, +11 authors T. Mak
  • Published 2005
  • Biology, Medicine
  • Cell
  • As components of the apoptosome, a caspase-activating complex, cytochrome c (Cyt c) and Apaf-1 are thought to play critical roles during apoptosis. Due to the obligate function of Cyt c in electron transport, its requirement for apoptosis in animals has been difficult to establish. We generated "knockin" mice expressing a mutant Cyt c (KA allele), which retains normal electron transfer function but fails to activate Apaf-1. Most KA/KA mice displayed embryonic or perinatal lethality caused by… CONTINUE READING
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