Species-specific assessment of Mycobacterium leprae in skin biopsies by in situ hybridization and polymerase chain reaction.

@article{Arnoldi1992SpeciesspecificAO,
  title={Species-specific assessment of Mycobacterium leprae in skin biopsies by in situ hybridization and polymerase chain reaction.},
  author={J{\"o}rg Arnoldi and Carsten Schl{\"u}ter and Michael Duchrow and L. W. Huebner and Maurice. Ernst and A. Larry Teske and Hans D. Flad and Johannes Gerdes and Erik C B{\"o}ttger},
  journal={Laboratory investigation; a journal of technical methods and pathology},
  year={1992},
  volume={66 5},
  pages={
          618-23
        }
}
Conventional histopathologic diagnosis of mycobacterial infections are limited to the determination of "acid-fast bacilli". A species-specific diagnosis is thus far impossible. In addition, routine microbiologic assessments of mycobacteria suffer from the major drawback that a species-specific diagnosis is extremely time-consuming and in several cases even impossible. As Mycobacterium leprae cannot be cultured in vitro, we tried to specifically target this obligate intracellular parasite by in… CONTINUE READING

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