Species-level identification of orthopoxviruses with an oligonucleotide microchip.

  title={Species-level identification of orthopoxviruses with an oligonucleotide microchip.},
  author={Sergey A Lapa and Maxim Mikheev and Sergei Nikolaevich Shchelkunov and Vladimir M. Mikhailovich and Alexander Sobolev and V. M. Blinov and Igor V. Babkin and Alexander Guskov and Elena Sokunova and A. S. Zasedatelev and L. S. Sandakhchiev and Andrei Mirzabekov},
  journal={Journal of clinical microbiology},
  volume={40 3},
A method for species-specific detection of orthopoxviruses pathogenic for humans and animals is described. The method is based on hybridization of a fluorescently labeled amplified DNA specimen with the oligonucleotide DNA probes immobilized on a microchip (MAGIChip). The probes identify species-specific sites within the crmB gene encoding the viral analogue of tumor necrosis factor receptor, one of the most important determinants of pathogenicity in this genus of viruses. The diagnostic… CONTINUE READING

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