Species-independent translational leaders facilitate cell-free expression

@article{Mureev2009SpeciesindependentTL,
  title={Species-independent translational leaders facilitate cell-free expression},
  author={Sergei Mureev and Oleksiy Kovtun and Uyen T. T. Nguyen and Kirill Alexandrov},
  journal={Nature Biotechnology},
  year={2009},
  volume={27},
  pages={747-752}
}
Cell-free protein synthesis enables the rapid production and engineering of recombinant proteins. Existing cell-free systems differ substantially from each other with respect to efficiency, scalability and the ability to produce functional eukaryotic proteins. Here we describe species-independent translational sequences (SITS) that mediate efficient cell-free protein synthesis in multiple prokaryotic and eukaryotic systems, presumably through bypassing the early translation initiation factors… CONTINUE READING

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