Species identification of animal cells by nested PCR targeted to mitochondrial DNA

  title={Species identification of animal cells by nested PCR targeted to mitochondrial DNA},
  author={Kazumi Ono and Motonobu Satoh and Touho Yoshida and Yutaka Ozawa and Arihiro Kohara and Masao Takeuchi and Hiroshi Mizusawa and Hidekazu Sawada},
  journal={In Vitro Cellular & Developmental Biology - Animal},
We developed a highly sensitive and convenient method of nested polymerase chain reaction (PCR) targeted to mitochondrial deoxyribonucleic acid (DNA) to identify animal species quickly in cultured cells. Fourteen vertebrate species, including human, cynomolgus monkey, African green monkey, mouse, rat, Syrian hamster, Chinese hamster, guinea pig, rabbit, dog, cat, cow, pig, and chicken, could be distinguished from each other by nested PCR. The first PCR amplifies mitochondrial DNA fragments with… CONTINUE READING
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