Species-dependent expression of the hyoscyamine 6 beta-hydroxylase gene in the pericycle.

@article{Kanegae1994SpeciesdependentEO,
  title={Species-dependent expression of the hyoscyamine 6 beta-hydroxylase gene in the pericycle.},
  author={Takeshi Kanegae and Hiromi Kajiya and Yasuhiro Amano and Takashi Hashimoto and Yasuyuki Yamada},
  journal={Plant physiology},
  year={1994},
  volume={105 2},
  pages={483-90}
}
The tropane alkaloid scopolamine is synthesized in the pericycle of branch roots in certain species of the Solanaceae. The enzyme responsible for the synthesis of scopolamine from hyoscyamine is hyoscyamine 6 beta-hydroxylase (H6H). The gene for H6H was isolated from Hyoscyamus niger. It has an exon/intron organization very similar to those for ethylene-forming enzymes, suggesting a common evolutionary origin. The 827-bp 5' flanking region of the H6H gene was fused to the beta-glucuronidase… CONTINUE READING

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The tropane alkaloid scopolamine is synthesized in the pericycle of branch roots in certain species of the Solanaceae .
The 827-bp 5 ' flanking region of the H6H gene was fused to the beta - glucuronidase ( GUS ) reporter gene and transferred to three solanaceous species by Agrobacterium - mediated transformation systems : H. niger and belladonna ( Atropa belladonna ) , which have high and low levels , respectively , of H6H mRNA in the root , and tobacco ( Nicotiana tabacum ) , which has no endogenous H6H gene .
The 827-bp 5 ' flanking region of the H6H gene was fused to the beta - glucuronidase ( GUS ) reporter gene and transferred to three solanaceous species by Agrobacterium - mediated transformation systems : H. niger and belladonna ( Atropa belladonna ) , which have high and low levels , respectively , of H6H mRNA in the root , and tobacco ( Nicotiana tabacum ) , which has no endogenous H6H gene .
The enzyme responsible for the synthesis of scopolamine from hyoscyamine is hyoscyamine 6 beta - hydroxylase ( H6H ) .
The enzyme responsible for the synthesis of scopolamine from hyoscyamine is hyoscyamine 6 beta - hydroxylase ( H6H ) .
The tropane alkaloid scopolamine is synthesized in the pericycle of branch roots in certain species of the Solanaceae .
Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of hairy roots and plants of transgenic tobacco .
Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of hairy roots and plants of transgenic tobacco .
Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of hairy roots and plants of transgenic tobacco .
Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of hairy roots and plants of transgenic tobacco .
Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of hairy roots and plants of transgenic tobacco .
Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of hairy roots and plants of transgenic tobacco .
Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of hairy roots and plants of transgenic tobacco .
Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of hairy roots and plants of transgenic tobacco .
Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of transgenic H. niger hairy roots , but only at the root meristem of hairy roots and plants of transgenic tobacco .
In transgenic hairy roots and regenerated plants of belladonna , the root meristem was stained with GUS activity , except for a few transformants in which the vascular cylinder was also stained .
The enzyme responsible for the synthesis of scopolamine from hyoscyamine is hyoscyamine 6 beta - hydroxylase ( H6H ) .
The enzyme responsible for the synthesis of scopolamine from hyoscyamine is hyoscyamine 6 beta - hydroxylase ( H6H ) .
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