Specialized Transducing Bacteriophage Lambda Carrying the PolC* Locus of Escherichia coli


We have isolated a specialized transducing phage carrying the poiC locus of E. coli K-12. A strain of E. coli lacking the lambda attachment site was infected with bacteriophage X. Lysogens carrying X at the tonA locus were isolated by selecting X-immune, T5-resistant strains. Transducing phages of dapC, dapD and polC, which map within 0.2 min of tonA, were… (More)


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