SpeB proteolysis with imaged capillary isoelectric focusing for the characterization of domain-specific charge heterogeneities of reference and biosimilar Rituximab.

Abstract

The charge variations of therapeutic monoclonal antibody reveal important information of the post-translational modifications that may potentially impact the potency and safety of pharmaceutical products, especially during the evaluation of biosimilarity of therapeutic proteins. In this work, a novel SpeB-based proteolysis strategy coupling with imaged… (More)
DOI: 10.1016/j.jchromb.2016.03.031

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@article{Zhang2016SpeBPW, title={SpeB proteolysis with imaged capillary isoelectric focusing for the characterization of domain-specific charge heterogeneities of reference and biosimilar Rituximab.}, author={Zichuan Zhang and Ronel Perrault and Yun Zhao and Julia H Ding}, journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences}, year={2016}, volume={1020}, pages={148-57} }