Spatiotemporal control of spindle midzone formation by PRC1 in human cells.

  title={Spatiotemporal control of spindle midzone formation by PRC1 in human cells.},
  author={Changjun Zhu and Eric Ho-Yin Lau and Robert Schwarzenbacher and Ella Bossy-Wetzel and Wei Jiang},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={103 16},
We have examined the role of PRC1, a midzone-associated, microtubule bundling, Cdk substrate protein, in regulating the spatiotemporal formation of the midzone in HeLa cells. Cdk-mediated phosphorylation of PRC1 in early mitosis holds PRC1 in an inactive monomeric state. During the metaphase-to-anaphase transition, PRC1 is dephosphorylated, promoting PRC1 oligomerization. Using time-lapse video microscopy, RNA interference, 3D immunofluorescence reconstruction imaging, and rescue experiments… CONTINUE READING

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