Spatiotemporal analysis of endocytosis and membrane distribution of fluorescent sterols in living cells

@article{Wstner2008SpatiotemporalAO,
  title={Spatiotemporal analysis of endocytosis and membrane distribution of fluorescent sterols in living cells},
  author={Daniel W{\"u}stner and Nils J F{\ae}rgeman},
  journal={Histochemistry and Cell Biology},
  year={2008},
  volume={130},
  pages={891-908}
}
Distribution and dynamics of cholesterol in the plasma membrane as well as internalization pathways for sterol from the cell surface are of great cell biological interest. Here, UV-sensitive wide field microscopy of the intrinsically fluorescent sterols, dehydroergosterol (DHE) and cholestatrienol (CTL) combined with advanced image analysis were used to study spatiotemporal sterol distribution in living macrophages, adipocytes and fibroblasts. Sterol endocytosis was directly visualized by time… CONTINUE READING
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