• Corpus ID: 55152016

Spatio-temporal modeling of morphology and gene-expressions during C. elegans embryogenesis using a new imaging framework

  title={Spatio-temporal modeling of morphology and gene-expressions during C. elegans embryogenesis using a new imaging framework},
  author={Johan Henriksson},
Many aspects of ontogenesis, the process whereby an organism develops from the fertilized egg to a grown adult, are still poorly understood, despite having been studied since Aristotle's time. The advent of modern biology with the sequencing of entire genomes and mapping of all genes offers the promise that one day we will understand how genes function to regulate cell division and differentiation. These advances offer new possibilities to solve the question that humanity has been asking since… 


Assessing normal embryogenesis in Caenorhabditis elegans using a 4D microscope: variability of development and regional specification.
It is postulate that the production of regions by cell-cell interactions is the pivotal principle guiding the embryogenesis of C. elegans and that the embryogenic of the worm follows the same basic principles as embryogenesis in other organisms.
High-Throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans
Using DNA sequences 5′ to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in
Genome-scale analysis of in vivo spatiotemporal promoter activity in Caenorhabditis elegans
Automated comparison and clustering of the obtained in vivo expression patterns show that genes coexpressed in space and time tend to belong to common functional categories, enabling prediction of anatomical and temporal interaction territories between protein partners.
C. elegans : a practical approach
The first chapter gives all the basic information on C. elegans required to use it: it's natural history, anatomy, life cycle, development, and evolution, and the essential practical details of transformation and common uses for the technique are given.
The Nematode Caenorhabditis elegans
This "Book of the Worm" serves as a reference source for C. elegans investigators as well as an introductory monograph for other biologists.
The embryonic cell lineage of the nematode Caenorhabditis elegans.
Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
It is found that genes of similar functions are clustered in distinct, multi-megabase regions of individual chromosomes; genes in these regions tend to share transcriptional profiles.
A 3D Digital Atlas of C. elegans and Its Application To Single-Cell Analyses
A digital nuclear atlas of the newly hatched, first larval stage (L1) of the wild-type hermaphrodite of Caenorhabditis elegans and an automated approach to assign cell names to each nucleus in a three-dimensional image of an L1 worm to allow high-throughput single-cell analyses of the post-embryonic worm.
PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans.
Here a protocol is described to create gfp fusion constructs that are ready for injection into the C. elegans gonad within one day, with no need for subcloning procedures, and is schematically outlined in Figure 1.
Automated analysis of embryonic gene expression with cellular resolution in C. elegans
We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility