Spatio-temporal kinetics of growth hormone receptor signaling in single cells using FRET microscopy.

@article{BienerRamanujan2006SpatiotemporalKO,
  title={Spatio-temporal kinetics of growth hormone receptor signaling in single cells using FRET microscopy.},
  author={Eva Biener-Ramanujan and Vivek Ramanujan and Brian A. Herman and Arieh Gertler},
  journal={Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society},
  year={2006},
  volume={16 4},
  pages={247-57}
}
The growth hormone (GH) receptor (R)-mediated JAK2 (Janus kinase-2)-STAT5 (signaling transducer and activator of transcription-5) pathway involves a cascade of protein-protein interactions and tyrosine phosphorylations that occur in a spatially and temporally sensitive manner in cells. To study GHR dimerization or GH-induced conformational change of predimerized GHRs and STAT5 activation kinetics in intact cells, fluorescence resonance energy transfer (FRET) and live-cell imaging methods were… CONTINUE READING