Spatially Restricted Expression of pipe in the Drosophila Egg Chamber Defines Embryonic Dorsal–Ventral Polarity

@article{Sen1998SpatiallyRE,
  title={Spatially Restricted Expression of pipe in the Drosophila Egg Chamber Defines Embryonic Dorsal–Ventral Polarity},
  author={J. Sen and J. Goltz and L. Stevens and D. Stein},
  journal={Cell},
  year={1998},
  volume={95},
  pages={471-481}
}
Expression of pipe in the somatic tissue of the Drosophila ovary is required for the formation of embryonic dorsal-ventral polarity. pipe, which encodes an enzyme similar to the glycosaminoglycan-modifying enzyme heparan sulfate 2-O-sulfotransferase, is expressed in a spatially restricted domain of follicle cells on the ventral side of the egg chamber. Mutations that affect follicular polarity correspondingly alter the spatial pattern of pipe expression. Directed expression of pipe in otherwise… Expand
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It is shown that this ventralization of the resulting embryos is dependent on the other members of the dorsal group of genes controlling dorsal-ventral polarity, but not on the state of the Epidermal Growth Factor Receptor signal transduction pathway which defines egg chamber polarity. Expand
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Findings support the view that the pipe domain is directly delimited by a long-range Gurken gradient and indicates that potent inhibitory processes prevent pipe dependent Toll activation at the dorsal side of the egg. Expand
Sulfation of Eggshell Components by Pipe Defines Dorsal-Ventral Polarity in the Drosophila Embryo
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It is demonstrated that dorsalized embryos are only produced by egg chambers in which both germline and follicle cells lack PAPS synthetase activity, which indicates the existence of a conduit for the movement of PAPS between the germlines and the follicles, which highlights a previously unappreciated mechanism of soma/germline cooperation affecting pattern formation. Expand
Maternal control of the Drosophila dorsal–ventral body axis
  • D. Stein, L. Stevens
  • Biology, Medicine
  • Wiley interdisciplinary reviews. Developmental biology
  • 2014
TLDR
Classic studies are reviewed and the details of more recent work that has advanced the understanding of the complex pathway that establishes Drosophila embryo DV polarity are integrated. Expand
Molecular mechanisms of EGF signaling-dependent regulation of pipe, a gene crucial for dorsoventral axis formation in Drosophila
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The analysis of mutant follicle cell clones reveals that none of the transcription factors known to act downstream of EGF signaling in Drosophila is required or sufficient for pipe regulation, however, the pipe cis-regulatory region harbors a 31-bp element which is essential for pipe repression, and ovarian extracts contain a protein that binds this element. Expand
No requirement for localized Nudel protein expression in Drosophila embryonic axis determination
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A mosaic analysis of additional nudel alleles found no requirement for nudel to be expressed in ventral, pipe-expressing follicle cells, thereby eliminating Nudel as an essential substrate of Pipe sulfotransferase activity. Expand
Regulation of dorso/ventral patterning in the drosophila embryo by multiple dorsal-interacting proteins
TLDR
The Rel family transcription factor, Dorsal, determines cell fate as a function of position along the dorsoventral axis of the Drosophila embryo using a broad array of regulatory interactions, serving as an excellent paradigm for eukaryotic transcriptional regulation. Expand
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