Spatially Restricted Expression of pipe in the Drosophila Egg Chamber Defines Embryonic Dorsal–Ventral Polarity

  title={Spatially Restricted Expression of pipe in the Drosophila Egg Chamber Defines Embryonic Dorsal–Ventral Polarity},
  author={Jonaki Sen and Jason S Goltz and Leslie M Stevens and David S. Stein},

Figures from this paper

Windbeutel is required for function and correct subcellular localization of the Drosophila patterning protein Pipe.

It is shown that this ventralization of the resulting embryos is dependent on the other members of the dorsal group of genes controlling dorsal-ventral polarity, but not on the state of the Epidermal Growth Factor Receptor signal transduction pathway which defines egg chamber polarity.

Establishment of dorsal-ventral polarity of the Drosophila egg requires capicua action in ovarian follicle cells.

The experiments reveal that cic controls dorsal-ventral patterning by regulating pipe expression in ovarian follicle cells, before its previously described role in interpreting the Dorsal gradient.

Distinct functional specificities are associated with protein isoforms encoded by the Drosophila dorsal-ventral patterning gene pipe

Uniform expression of the Pipe-ST2 isoform in the follicle cell layer of females otherwise lacking pipe expression leads to the formation of embryos with a DV axis that is appropriately oriented with respect to the intrinsic polarity of the eggshell, suggesting the existence of a second mechanism that polarizes the Drosophila embryo.

Mechanisms of Gurken-dependent pipe regulation and the robustness of dorsoventral patterning in Drosophila.

Findings support the view that the pipe domain is directly delimited by a long-range Gurken gradient and indicates that potent inhibitory processes prevent pipe dependent Toll activation at the dorsal side of the egg.

Maternal control of the Drosophila dorsal–ventral body axis

Classic studies are reviewed and the details of more recent work that has advanced the understanding of the complex pathway that establishes Drosophila embryo DV polarity are integrated.

Synthesis of the sulfate donor PAPS in either the Drosophila germline or somatic follicle cells can support embryonic dorsal-ventral axis formation

It is demonstrated that dorsalized embryos are only produced by egg chambers in which both germline and follicle cells lack PAPS synthetase activity, which indicates the existence of a conduit for the movement of PAPS between the germlines and the follicles, which highlights a previously unappreciated mechanism of soma/germline cooperation affecting pattern formation.

Molecular mechanisms of EGF signaling-dependent regulation of pipe, a gene crucial for dorsoventral axis formation in Drosophila

The analysis of mutant follicle cell clones reveals that none of the transcription factors known to act downstream of EGF signaling in Drosophila is required or sufficient for pipe regulation, but the pipe cis-regulatory region harbors a 31-bp element which is essential for pipe repression, and ovarian extracts contain a protein that binds this element.

No requirement for localized Nudel protein expression in Drosophila embryonic axis determination

A mosaic analysis of additional nudel alleles found no requirement for nudel to be expressed in ventral, pipe-expressing follicle cells, thereby eliminating Nudel as an essential substrate of Pipe sulfotransferase activity.

Regulation of dorso/ventral patterning in the drosophila embryo by multiple dorsal-interacting proteins

The Rel family transcription factor, Dorsal, determines cell fate as a function of position along the dorsoventral axis of the Drosophila embryo using a broad array of regulatory interactions, serving as an excellent paradigm for eukaryotic transcriptional regulation.



Mechanisms of dorsal-ventral axis determination in Drosophila embryos revealed by cytoplasmic transplantations.

The results of single and double injections suggest that the spatial information for the embryonic dorsal-ventral axis is largely derived from spatial cues present in the extraembryonic compartment, which restrict the release of the putative Toll ligand.

The relationship between ovarian and embryonic dorsoventral patterning in Drosophila.

The results imply that gurken, unlike other localized cytoplasmic determinants, is not directly responsible for the establishment of cell fates along a body axis, but that it restricts and orients an active axis-forming process which occurs later in the follicular epithelium or in the early embryo.

torso-like encodes the localized determinant of Drosophila terminal pattern formation.

Ectopic expression of tsl produced embryos with a phenotype similar to that resulting from constitutively active Tor alleles, suggesting that localized TSL controls the localized activation of TOR.

Signaling pathways that establish the dorsal-ventral pattern of the Drosophila embryo.

The dorsal-ventral pattern of the Drosophila embryo is established by three sequential signaling pathways, each of which uses a distinct strategy to achieve spatial localization of signaling activity.