Spatial light interference microscopy: principle and applications to biomedicine.
@article{Chen2020SpatialLI,
title={Spatial light interference microscopy: principle and applications to biomedicine.},
author={Xi Chen and Mikhail Eugene Kandel and Gabriel Popescu},
journal={Advances in optics and photonics},
year={2020},
volume={13 2},
pages={
353-425
}
}In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase imaging (QPI) methods, SLIM allows for speckle-free phase reconstruction with sub-nanometer path-length stability. We first review image formation in QPI, scattering, and full-field methods. Then, we outline SLIM imaging from theory and instrumentation to…
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264 References
Spatial light interference microscopy (SLIM)
- BiologyIEEE Photonic Society 24th Annual Meeting
- 2011
Spatial light interference microscopy reveals the intrinsic contrast of cell structures and renders quantitative optical path-length maps across the sample, which may prove instrumental in impacting the light microscopy field at a large scale.
Fourier phase microscopy with white light.
- PhysicsBiomedical optics express
- 2013
WFPM is presented with spatially coherent white light (wFPM), which offers high spatial phase sensitivity due to the low temporal coherence and high temporal phase stability due to common path geometry.
Cardiomyocyte Imaging Using Real-Time Spatial Light Interference Microscopy (SLIM)
- PhysicsPloS one
- 2013
This paper presents the dispersion relation, i.e. decay rate vs. spatial mode, associated with dynamic beating cardiomyocyte cells from the quantitative phase images obtained with the real-time SLIM system, and uses a fast LCPM for phase shifting and a fast scientific-grade complementary metal oxide semiconductor camera (sCMOS) camera (Andor) for imaging.
Label-free quantitative 3D tomographic imaging for partially coherent light microscopy.
- PhysicsOptics express
- 2017
The PC-ODT can be considered as an efficient and affordable alternative to coherent ODT which requires specially designed holographic microscopes and can be easily implemented in commercially available bright-field microscopes.
White-light diffraction phase microscopy at doubled space-bandwidth product.
- PhysicsOptics express
- 2016
White light diffraction microscopy (wDPM) is a quantitative phase imaging method that benefits from both temporal and spatial phase sensitivity, granted, respectively, by the common-path geometry and…
Super-Resolved Spatial Light Interference Microscopy
- Physics
In this report, structured illumination applications for spatial light interference microscopy (SLIM), based on the study titled " Super-Resolved Spatial Light Interference Microscopy " by Chu et…
White-light diffraction tomography of unlabelled live cells
- MedicineNature Photonics
- 2014
The results establish white-light diffraction tomography as a means for measuring three-dimensional subcellular structures in a non-invasive and label-free manner.
White-light quantitative phase imaging unit.
- PhysicsOptics express
- 2016
With speckle-free imaging capability due to the use of white-light illumination, the WQPIU is expected to expand the scope of QPI in biological sciences as a powerful but simple imaging tool.
Three-dimensional quantitative phase imaging via tomographic deconvolution phase microscopy.
- PhysicsApplied optics
- 2015
A new phase reconstruction method, called tomographic deconvolution phase microscopy (TDPM), is described which makes use of commercial microscopy hardware in realizing 3D QPI and is based on the 3D weak object transfer function theory, shown here to be capable of imaging "nonweak" phase objects with large phase excursions.
Label-free Rheinberg staining of cells using digital holographic microscopy and spatial light interference microscopy
- BiologySPIE/COS Photonics Asia
- 2019
This paper develops a theory for image formation with Rheinberg illumination under the conditions of Kohler illumination and reviews and develops this approach by testing it with multiple different modalities for recording the QPI image, namely digital holographic microscopy, which uses coherent illumination and spatial light interference microscopy.