Spatial frequency filtering and its application to microscopy.

  title={Spatial frequency filtering and its application to microscopy.},
  author={Peter J.S Hutzler},
  journal={Applied optics},
  volume={16 8},
Application of spatial frequency filtering techniques gives powerful tools for the automation of screening ofbiomedical microsamples. Band-pass filtering provides a size selection of objects, whereas high-pass filtering combined with a subsequent reduction of the field size enables the separation of the superposition of thefield and the object spectrum. Besides the theoretical background and numerical evaluations, experimental applications to cytological and cytogenetical samples are shown.