Spatial distribution and diffusive motion of RNA polymerase in live Escherichia coli.

  title={Spatial distribution and diffusive motion of RNA polymerase in live Escherichia coli.},
  author={Benjamin P Bratton and R A Mooney and James C Weisshaar},
  journal={Journal of bacteriology},
  volume={193 19},
By labeling the β' subunit of RNA polymerase (RNAP), we used fluorescence microscopy to study the spatial distribution and diffusive motion of RNAP in live Escherichia coli cells for the first time. With a 40-ms time resolution, the spatial distribution exhibits two or three narrow peaks of 300- to 600-nm full width at half-maximum that maintain their positions within 60 nm over 1 s. The intensity in these features is 20 to 30% of the total. Fluorescence recovery after photobleaching (FRAP… CONTINUE READING

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