Spatial control of coated-pit dynamics in living cells

  title={Spatial control of coated-pit dynamics in living cells},
  author={I. Gaidarov and F. Santini and R. A. Warren and J. Keen},
  journal={Nature Cell Biology},
  • I. Gaidarov, F. Santini, +1 author J. Keen
  • Published 1999
  • Biology, Medicine
  • Nature Cell Biology
  • Here we visualize new aspects of the dynamics of endocytotic clathrin-coated pits and vesicles in mammalian cells by using a fusion protein consisting of green fluorescent protein and clathrin light chain a. Clathrin-coated pits invaginating from the plasma membrane show definite, but highly limited, mobility within the membrane that is relaxed upon treatment with latrunculin B, an inhibitor of actin assembly, indicating that an actin-based framework may be involved in the mobility of these… CONTINUE READING
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