Spatial control of coated-pit dynamics in living cells

  title={Spatial control of coated-pit dynamics in living cells},
  author={Ibragim Gaidarov and Francesca Santini and Robin A. Warren and James H. Keen},
  journal={Nature Cell Biology},
Here we visualize new aspects of the dynamics of endocytotic clathrin-coated pits and vesicles in mammalian cells by using a fusion protein consisting of green fluorescent protein and clathrin light chain a. Clathrin-coated pits invaginating from the plasma membrane show definite, but highly limited, mobility within the membrane that is relaxed upon treatment with latrunculin B, an inhibitor of actin assembly, indicating that an actin-based framework may be involved in the mobility of these… 

Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits

Evanescent field microscopy is used to observe single clathrin-coated pits or vesicles as they draw inwards from the plasma membrane and finally lose their coats, suggesting dynamin may provide the trigger and actin may provided the force for movement into the cytosol.

Dynamics and Cargo Selectivity of Endocytic Adaptor Proteins

It is demonstrated that the dynamic population of clathrin is primarily endosomal, as it lacks colocalization with the plasma membrane-specific endocytic adaptor AP-2, but colocalizes with large, internalized low density lipoprotein (LDL) and transferrin positive structures.

Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network.

The use of fluorescent imaging of live cells indicates the existence of a novel type of trans-Golgi network-derived carriers containing associated clathrin, GGA1 and adaptor protein-1 that are larger than conventionalClathrin-coated vesicles, and that undergo long-range translocation in the cytoplasm before losing their coats.

Understanding Living Clathrin‐Coated Pits

The direct observation of CCP‐associated proteins including clathrin itself, dynamin and, most recently, AP‐2 has considerably challenged old models, confirming some points but raising very interesting new questions.



Endocytic clathrin-coated pit formation is independent of receptor internalization signal levels.

The results provide a clear indication that receptor endocytic signals do not determine coated pit levels by directly recruiting AP2 molecules, and support a model in which coated pit formation proceeds through recruitment and activation of AP2, likely through a limited number of regulated docking sites that act independently of endocytical signals.

Clathrin-coated vesicle formation and protein sorting: an integrated process.

  • S. Schmid
  • Biology, Chemistry
    Annual review of biochemistry
  • 1997
The functional interplay between the coated vesicle machinery and its cargo could ensure sorting fidelity and packaging efficiency and might enable modulation of vesicular trafficking in response to demand.

The role of clathrin, adaptors and dynamin in endocytosis.

  • M. Robinson
  • Biology, Chemistry
    Current opinion in cell biology
  • 1994

A novel class of clathrin-coated vesicles budding from endosomes

Transferrin receptor recycling as well as the formation of clathrin-coated pits at endosomes was inhibited in the presence of brefeldin A, which indicates that a novel class of clATHrin- coated vesicles serves an exit pathway from endosome-associated buds.

Dissecting clathrin-coated pits.

Three-dimensional visualization of coated vesicle formation in fibroblasts

  • J. Heuser
  • Biology
    The Journal of cell biology
  • 1980
The quick-freeze, deep- etch, rotary-replication method has been applied to fibroblasts and displays with unusual clarity the coats that appear under the plasma membrane at the start of receptor-mediated endocytosis, including the most noteworthy variations in the typical hexagonal honeycomb.

Topological mechanisms involved in the formation of clathrin-coated vesicles.

Role of coated vesicles, microfilaments, and calmodulin in receptor- mediated endocytosis by cultured B lymphoblastoid cells

The pathways of endocytosis in this cell are clarified and functional roles for calmodulin, especially in the formation of clathrin-coated pits, and for actin microfilaments in coated vesicle formation and in capping are suggested.

Endocytosis of activated receptors and clathrin-coated pit formation: deciphering the chicken or egg relationship

Results indicate that de novo formation of clathrin-coated pits is not a prerequisite for rapid internalization or a direct response to stimulation of FCepsilonRI receptors, and increases in coated pits reported to occur in response to activation of some signaling receptors must be consequences of the signal transduction processes, rather than strictly serving the purpose of the internalization of the receptors.