Spatial and temporal nature of reactive oxygen species production and programmed cell death in elm (Ulmus pumila L.) seeds during controlled deterioration.

@article{Hu2012SpatialAT,
  title={Spatial and temporal nature of reactive oxygen species production and programmed cell death in elm (Ulmus pumila L.) seeds during controlled deterioration.},
  author={Die Hu and Gang Ma and Qiong Wang and Jinghan Yao and Yu Wang and Hugh W. Pritchard and Xiaofeng Wang},
  journal={Plant, cell & environment},
  year={2012},
  volume={35 11},
  pages={2045-59}
}
Seed deterioration is poorly understood and remains an active area for research. Seeds of elm (Ulmus pumila L.) were aged at 37 °C above water [controlled deterioration treatment (CDT)] for various lengths of time to assess programmed cell death (PCD) and reactive oxygen species (ROS) product in embryonic tissues during a 5 d period. The hallmarks of PCD were identified in the elm seeds during CDT including TUNEL experiments, DNA laddering, cytochrome c (cyt c) leakage and enzymatic activities… CONTINUE READING
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