Spatial and temporal dynamics of the microbial community in the Hanford unconfined aquifer

  title={Spatial and temporal dynamics of the microbial community in the Hanford unconfined aquifer},
  author={Xueju Lin and J. McKinley and Charles Tom Resch and Rachael Kaluzny and Christian L. Lauber and James K. Fredrickson and Rob Knight and Allan Konopka},
  journal={The ISME Journal},
Pyrosequencing analysis of 16S rRNA genes was used to study temporal dynamics of groundwater bacteria and archaea over 10 months within three well clusters separated by ∼30 m and located 250 m from the Columbia River on the Hanford Site, WA. Each cluster contained three wells screened at different depths ranging from 10 to 17 m that differed in hydraulic conductivities. Representative samples were selected for analyses of prokaryotic 16S and eukaryotic 18S rRNA gene copy numbers. Temporal… CONTINUE READING
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