Sparsomycin Antibiotic Production by Streptomyces Sp. AZ-NIOFD1: Taxonomy, Fermentation, Purification and Biological Activities

Abstract

This work was carried out in the course of a screening program for specific the bioactive substances that demonstrated inhibitory affects against prokaryotic and eukaryotic microorganisms from actinomycetes strains..Five actinomycete isolates could be isolated from water sample collected from River Nile, Egypt were screened for antimicrobial activities in starch nitrate medium. One of the actinomycete cultures (AZ-NIOFD ) 1 was found to produce a wide spectrum antimicrobial agent. The most potent of the producer strains was selected and identified. The cultural, physiological and biochemical characteristics of the isolate identified the strain as a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene (1.5 kb) of the most potent strain evidenced a 98% similarity with S. tritolerans and a 96% similarity with S. plicatus 16 rRNA genes and the isolated strain was ultimately identified as Streptomyces sp. MAR01. The extraction S of the fermentation broth of this strain resulted in the isolation of one major compound, which was active in vitro against Gram-positive, Gram-negative bacteria and unicellular and filamentous fungi. The physico-chemical characteristics of the purified antibiotic viz. color, melting point, solubility, elemental analysis, spectroscopic characteristics and chemical reactions have been investigated. This analysis indicates a suggested imperical formula of C H N O S . The minimum inhibition concentrations "MICs" of the purified 13 21 3 6 2 antibiotic were also determined. In conclusion, the collected data emphasized the fact that the purified antibiotic compound was suggestive of being belonging to sparsomycin antibiotic produced by Streptomyces violaceusniger, AZ-NIOFD . 1

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@inproceedings{Atta2013SparsomycinAP, title={Sparsomycin Antibiotic Production by Streptomyces Sp. AZ-NIOFD1: Taxonomy, Fermentation, Purification and Biological Activities}, author={Houssam M. Atta and Sherif M. Dabour}, year={2013} }