Sparged animal cell bioreactors: mechanism of cell damage and Pluronic F-68 protection.

@article{Murhammer1990SpargedAC,
  title={Sparged animal cell bioreactors: mechanism of cell damage and Pluronic F-68 protection.},
  author={David W. Murhammer and Charles F. Goochee},
  journal={Biotechnology progress},
  year={1990},
  volume={6 5},
  pages={391-7}
}
Pluronic F-68 is a widely used protective agent in sparged animal cell bioreactors. In this study, the attachment-independent Spodoptera frugiperda Sf9 insect cell line was used to explore the mechanism of this protective effect and the nature of cell damage in sparged bioreactors. First, bubble incorporation via cavitation or vortexing was induced by increasing the agitation rate in a surface-aerated bioreactor; insect cells were rapidly killed under these conditions of the absence of polyols… CONTINUE READING

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