Spacing between GT-1 binding sites within a light-responsive element is critical for transcriptional activity.

@article{Gilmartin1990SpacingBG,
  title={Spacing between GT-1 binding sites within a light-responsive element is critical for transcriptional activity.},
  author={Philip M. Gilmartin and Nam Hai Chua},
  journal={The Plant cell},
  year={1990},
  volume={2 5},
  pages={447-55}
}
Dissection of the light-responsive element (LRE) located between -166 and -50 of rbcS-3A from pea has revealed critical spacing requirements between the two GT-1 binding sites for light-responsive transcription. An increase in spacing between the two sites by as little as 2 bp reduces dramatically the rbcS-3A transcript levels in vivo. Mutation of the 10 bp between the binding sites leads to slightly lower transcript levels, as do deletions of either 3 bp or 8 bp. Deletions of 5 bp or 7 bp from… CONTINUE READING