Spacer Asn determines the fate of Kunitz (STI) inhibitors, as revealed by structural and biochemical studies on WCI mutants.

@article{Dasgupta2006SpacerAD,
  title={Spacer Asn determines the fate of Kunitz (STI) inhibitors, as revealed by structural and biochemical studies on WCI mutants.},
  author={Jhimli Dasgupta and Susmita Khamrui and Jiban Kanti Dattagupta and Udayaditya Sen},
  journal={Biochemistry},
  year={2006},
  volume={45 22},
  pages={6783-92}
}
The scaffold of serine protease inhibitors plays a significant role in the process of religation which resists proteolysis of the inhibitor in comparison to a substrate. Although the role of the conserved scaffolding Asn residue was previously implicated in the maintenance of the binding loop conformation of Kunitz (STI) inhibitors, its possible involvement in the prevention of proteolysis is still unexplored. In this paper, we have investigated the specific role of the spacer Asn in the… CONTINUE READING