Sp17 gene expression in myeloma cells is regulated by promoter methylation

@article{Wang2004Sp17GE,
  title={Sp17 gene expression in myeloma cells is regulated by promoter methylation},
  author={Zhiqing Wang and Yansheng Zhang and Bernard Ramsahoye and David Q. Bowen and Seah H Lim},
  journal={British Journal of Cancer},
  year={2004},
  volume={91},
  pages={1597 - 1603}
}
The mechanisms underlying sperm protein 17 (Sp17) gene expression in myeloma cells remained unclear. Using reverse transcription–polymerase chain reaction (RT–PCR), Sp17 transcripts were detected in ARK-B, ARP-1, RPMI-8226 and KMS-11 but not in H929, IM-9, MM1-R and U266 cells. Using a panel of primer pairs in methylation-sensitive PCR to amplify overlapping gene segments, our screening studies showed that the HpaII sites at −359 and −350 are involved in the regulation of Sp17 gene expression… CONTINUE READING

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Sperm protein 17 (Sp17) as a target for immunotherapy of ovarian cancer

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