Soybean metal-binding proteins: calmodulin purification by hydrophobic interaction with polymer 3520.

  title={Soybean metal-binding proteins: calmodulin purification by hydrophobic interaction with polymer 3520.},
  author={L. C. Wang and R. E. Peterson and K. Shang and A. Ho and J. A. Rothfus},
  journal={Preparative biochemistry},
  volume={19 1},
Polymer 3520, a non-polar styrene divinylbenzene polymer, provides a simple way to purify calmodulin (CAM) from soybeans. This polymer, which selectively adsorbs CAM by hydrophobic interaction within the polymer matrix, contains no exchangeable groups; thus, interaction with CAM requires no Ca++ ions, and elution is achieved with 50% ethanol. Purification by this form of reversed-phase liquid chromatography is a substantial improvement over the conventional method, which requires high salt in… Expand
1 Citations
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