• Corpus ID: 46613566

Sox 2 Biology and Role in Development and Disease

@inproceedings{Kondoh2015Sox2B,
  title={Sox 2 Biology and Role in Development and Disease},
  author={Hisato Kondoh and Robin Lovell-Badge},
  year={2015}
}

Figures from this paper

References

SHOWING 1-10 OF 22 REFERENCES

Detecting transient intermediates in macromolecular binding by paramagnetic NMR

It is shown that intermolecular paramagnetic relaxation enhancement (PRE) provides a means of directly detecting the presence of, and investigating the nature of, low population transient intermediates under equilibrium conditions.

Direct observation of enhanced translocation of a homeodomain between DNA cognate sites by NMR exchange spectroscopy.

Rapid translocation at high concentrations of free DNA observed directly by NMR exchange spectroscopy reconciles the long half-lives of protein-DNA complexes measured by biochemical analysis in vitro with the highly dynamic behavior of such complexes observed in vivo.

Exploring translocation of proteins on DNA by NMR

Recent NMR developments from the laboratory that have permitted direct characterization of the species and molecular mechanisms involved in the target search process, including the detection of highly transient sparsely-populated states are summarized.

Reaction Rates by Nuclear Magnetic Resonance

The Bloch equations for nuclear magnetic resonance are modified to describe the magnetic resonance of a single nuclear species X which is transferred back and forth between two (or more) magnetic

Interplay between Minor and Major Groove-binding Transcription Factors Sox2 and Oct1 in Translocation on DNA Studied by Paramagnetic and Diamagnetic NMR*

Using NMR, the existence of sparsely populated, transient states that facilitate target location in Sox2·DNA and Oct1·Sox2· DNA complexes is demonstrated and Sox2 modulates the mechanism of translocation of Oct1.

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.

This work has shown that highly populated states are located within the global free energy minimum region of a relatively rough free energy hypersurface, and the structure and dynamics of lowly populated, higher free energy states which cannot be modeled.

Pairing SOX off: with partners in the regulation of embryonic development.

Impact of Protein/Protein Interactions on Global Intermolecular Translocation Rates of the Transcription Factors Sox2 and Oct1 between DNA Cognate Sites Analyzed by z-Exchange NMR Spectroscopy*

The kinetics of global intermolecular translocation of Sox2 and Oct1 between cognate sites located on different DNA molecules by z-exchange NMR spectroscopy suggest a model for the sequence of binding events involved in synergistic gene regulation by Sox2and Oct1.

Coevolution of HMG domains and homeodomains and the generation of transcriptional regulation by Sox/POU complexes

This review summarizes several examples of different HMG‐homeodomain interactions that illustrate not only the ancient origin of each of these protein families, but also their relationship to each other, and discusses how coevolution of HMG and homeodomains may have lead to creation of the specialized Sox/POU protein complexes.