Southern blot and dot blot hybridisation compared to PCR for the detection of human papillomavirus DNA in biopsies of the uterine cervix from women with dysplasia.

@article{Chen1993SouthernBA,
  title={Southern blot and dot blot hybridisation compared to PCR for the detection of human papillomavirus DNA in biopsies of the uterine cervix from women with dysplasia.},
  author={Sabrina M Chen and Christopher K Fairley and Sepehr N Tabrizi and Michael A. Quinn and Suzanne Marie Garland},
  journal={Clinical and diagnostic virology},
  year={1993},
  volume={1 3},
  pages={187-94}
}
The aim of this study was to compare the sensitivity of Southern blot (SB) and dot blot (DB) hybridisation with polymerase chain reaction (PCR) for the detection of HPV in cervical biopsies from samples with differing histology. One hundred and forty seven women with cervical dysplasia had biopsies performed; one sample was analyzed for HPV DNA from types 6/11, 16, and 18 by SB, DB and PCR (L1 consensus primer and type specific probes) while an adjacent sample was examined histologically. The… CONTINUE READING