Southern Blotting

  title={Southern Blotting},
  author={Terry Brown},
  journal={Current Protocols in Immunology},
  • T. Brown
  • Published 1 June 1993
  • Biology
  • Current Protocols in Immunology
Southern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in immobilization of the DNA fragments, so the membrane carries a semipermanent reproduction of the banding pattern of the gel. After immobilization, the DNA can be subjected to hybridization analysis, enabling bands with sequence similarity to a labeled probe to be identified. This unit describes Southern blotting via upward capillary transfer of DNA from an agarose gel onto a nylon… 

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Rapid transfer of DNA from agarose gels to nylon membranes.

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