Southern Blotting
@article{Brown2001SouthernB, title={Southern Blotting}, author={T. Brown}, journal={Current Protocols in Immunology}, year={2001}, volume={6} }
Southern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in immobilization of the DNA fragments, so the membrane carries a semipermanent reproduction of the banding pattern of the gel. After immobilization, the DNA can be subjected to hybridization analysis, enabling bands with sequence similarity to a labeled probe to be identified. This unit describes Southern blotting via upward capillary transfer of DNA from an agarose gel onto a nylon… CONTINUE READING
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References
SHOWING 1-10 OF 17 REFERENCES
Rapid transfer of DNA from agarose gels to nylon membranes.
- Biology, Medicine
- Nucleic acids research
- 1985
- 1,473
The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
- Chemistry, Medicine
- Analytical biochemistry
- 1980
- 567
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
- Biology, Medicine
- Proceedings of the National Academy of Sciences of the United States of America
- 1979
- 47,551
- PDF
One-hour downward alkaline capillary transfer for blotting of DNA and RNA.
- Chemistry, Medicine
- Analytical biochemistry
- 1992
- 544
- Highly Influential
Optimized Hybridization of DNA Blotted and Fixed to Nitrocellulose and Nylon Membranes
- Chemistry
- Bio/Technology
- 1987
- 166
Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.
- Biology, Medicine
- Nucleic acids research
- 1980
- 112
- PDF
Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose.
- Chemistry, Medicine
- Analytical biochemistry
- 1984
- 26
Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets.
- Chemistry, Medicine
- Analytical biochemistry
- 1980
- 360
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
- Biology, Medicine
- Journal of molecular biology
- 1975
- 25,164
- PDF