Sorting specimen-rich invertebrate samples with cost-effective NGS barcodes: Validating a reverse workflow for specimen processing.

@article{Wang2018SortingSI,
  title={Sorting specimen-rich invertebrate samples with cost-effective NGS barcodes: Validating a reverse workflow for specimen processing.},
  author={Wendy Y Wang and A. Srivathsan and Maosheng Foo and Seiki K Yamane and Rudolf Meier},
  journal={Molecular ecology resources},
  year={2018},
  volume={18 3},
  pages={
          490-501
        }
}
Biologists frequently sort specimen-rich samples to species. This process is daunting when based on morphology, and disadvantageous if performed using molecular methods that destroy vouchers (e.g., metabarcoding). An alternative is barcoding every specimen in a bulk sample and then presorting the specimens using DNA barcodes, thus mitigating downstream morphological work on presorted units. Such a "reverse workflow" is too expensive using Sanger sequencing, but we here demonstrate that is… CONTINUE READING

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