Corpus ID: 90444700

Sorting positive transformants from natural transformation in soil with flow cytometer

@inproceedings{Yuping2016SortingPT,
  title={Sorting positive transformants from natural transformation in soil with flow cytometer},
  author={Wang Yu-ping and Huang Qiao-yun and Chen Wen-li},
  year={2016}
}
  • Wang Yu-ping, Huang Qiao-yun, Chen Wen-li
  • Published 2016
  • Chemistry
  • One plasmid DNA with egfp and Kanr was introduced into the natural soil.The positive transformants were isolated directly from the bacterial suspension with flow cytometer and sorted with green fluorescence and kanamycin resistance.The frequency of natural transformation was less than 10 -7 in soil.After the enrich cultivation,82 cells out of 39 736 426 were sorted.Positive transformants were finally obtained.The occurrence of horizontal gene transfer through natural transformation in soil was… CONTINUE READING

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