Sonoporation of cultured cells in the rotating tube exposure system.

@article{Miller1999SonoporationOC,
  title={Sonoporation of cultured cells in the rotating tube exposure system.},
  author={Douglas L. Miller and Shiping Bao and James E. Morris},
  journal={Ultrasound in medicine & biology},
  year={1999},
  volume={25 1},
  pages={
          143-9
        }
}
Suspensions of Chinese hamster ovary cells were exposed to ultrasound in the presence of fluorescent dextran to determine the conditions needed for sonoporation with uptake of the large molecules. Albunex, a gas-body- based ultrasound contrast agent, was added to enhance cavitation. Ultrasound was continuous wave at frequencies of 1.0, 1.68, 2.25, 3.3, 5.3, and 7.15 MHz. Sterile 4.5-mL exposure chambers were rotated at 60 rpm to promote cavitation activity during the 1-min exposures. After… CONTINUE READING
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