Sonication assisted Agrobacterium-mediated transformation enhances the transformation efficiency in flax (Linum usitatissimum L.)

@article{Beranov2007SonicationAA,
  title={Sonication assisted Agrobacterium-mediated transformation enhances the transformation efficiency in flax (Linum usitatissimum L.)},
  author={Martina Beranov{\'a} and Slavom{\'i}r Rakousk{\'y} and Zuzana V{\'a}vrov{\'a} and Tom{\'a}s Skalick{\'y}},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2007},
  volume={94},
  pages={253-259}
}
A sonication-assisted, Agrobacterium-mediated, co-cultivation technique was used in an attempt to increase the transformation efficiency of flax. Hypocotyls and cotyledons excised from about 10-day-old flax seedlings grown in vitro were placed into a 10 mM MgSO4 solution, and inoculated with an A. tumefaciens vector bearing the mgfp5-ER gene driven by the CaMV 35S promoter. The explants were subjected to pulses of ultrasound delivered by a sonicator apparatus (35 kHz) for 0–150 s and co… CONTINUE READING