Some properties of the thiamine uptake system in isolated rat hepatocytes.

@article{Yoshioka1984SomePO,
  title={Some properties of the thiamine uptake system in isolated rat hepatocytes.},
  author={K. Yoshioka},
  journal={Biochimica et biophysica acta},
  year={1984},
  volume={778 1},
  pages={
          201-9
        }
}
  • K. Yoshioka
  • Published 1984
  • Chemistry, Medicine
  • Biochimica et biophysica acta
A kinetic study of [14C]thiamine uptake over a concentration range from 0.1 microM to 4 mM was performed in isolated rat hepatocytes. The results showed that two processes contribute to the entry in rat hepatocytes: a low affinity process with a Kt of 34.1 microM and Vmax of 20.8 pmol/10(5) cells per 30 s and a high affinity process with a Kt of 1.26 microM and Vmax of 1.21 pmol/10(5) cells per 30 s. The uptake of thiamine by the high affinity process was concentrative and reduced in a betaine… Expand
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References

SHOWING 1-10 OF 11 REFERENCES
Transport and metabolism of thiamin in isolated rat hepatocytes.
  • 37
  • PDF
Active transport of thiamine by freshly isolated rat hepatocytes.
  • C. Chen
  • Chemistry, Medicine
  • Journal of nutritional science and vitaminology
  • 1978
  • 25
Active transport of dimethialium in isolated rat hepatocytes.
  • 9
Intestinal absorption of choline in the chick.
  • 40
An improved method for the electrophoretic separation and fluorometric determination of thiamine and its phosphates in animal tissues.
  • C. Patrini, G. Rindi
  • Biology, Medicine
  • International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition
  • 1980
  • 29
Preparation of rat liver cells. I. Effect of Ca 2+ on enzymatic dispersion of isolated, perfused liver.
  • P. Seglen
  • Biology, Medicine
  • Experimental cell research
  • 1972
  • 462
Bacterial metabolism of thiamine. II. The isolation and characterization of 3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methylthiazole-5-acetic acid (thiamine acetic acid) as an intermediate in the oxidation of thiamine.
  • R. Neal
  • Biology, Medicine
  • The Journal of biological chemistry
  • 1969
  • 10
  • PDF
[16] Electrophoretic separation and fluorometric determination of thiamine and its phosphate esters
  • 29
Thiamine monophosphate, a normal constituent of rat plasma.
  • 22
STUDIES ON ENZYMATIC SYNTHESIS OF COCARBOXYLASE IN ANIMAL TISSUES:I. FUNDAMENTAL PROPERTIES OF THE REACTION
  • 9
...
1
2
...