Some properties of a cyclomaltodextrin-glucanotransferase from Bacillus circulans DF 9 R type.

@article{Marchal1996SomePO,
  title={Some properties of a cyclomaltodextrin-glucanotransferase from Bacillus circulans DF 9 R type.},
  author={Laurence Mar{\'e}chal and Adriana Mabel Rosso and Michel Andr{\'e} Mar{\'e}chal and Norberto Krymkiewicz and Susana A Ferrarotti},
  journal={Cellular and molecular biology},
  year={1996},
  volume={42 5},
  pages={659-64}
}
The cyclomaltodextrin-glucanotransferase (EC2.4.1.19, CGTase) which was purified to homogeneity from Bacillus circulans strain DF 9, R type, showed a pI of 5.3 determined by disc-isoelectric focusing, a Mw of 78 kDa estimated by SDS-PAGE with a range of pH of optimal enzymatic activity rather large (4.5-7.5). The thermal stability of the enzyme at 55 degrees C was increased 4-5 times when calcium ion (10 to 100 mM) or alpha-cyclodextrins (10 mM) were added to the preincubation mixtures. The… CONTINUE READING