Some kinetic studies on cytidine aminohydrolase activity from Aspergillus niger NRRL3.

@article{Ali1998SomeKS,
  title={Some kinetic studies on cytidine aminohydrolase activity from Aspergillus niger NRRL3.},
  author={Thanaa H. Ali},
  journal={Acta microbiologica Polonica},
  year={1998},
  volume={47 4},
  pages={
          365-72
        }
}
Cell free extract of nitrate-grown Aspergillus niger NRRL3 catalyzed the hydrolytic deamination of cytidine out of the tested bases, their nucleosides and nucleotides to uridine maximally at pH 7 and at 50 degrees C. The deaminating activity seems to be specific for cytidine, as the extracts could not deaminate AMP, GMP, CMP, adenosine, guanosine, adenine, guanine, and cytosine. Maximum activity of cytidine deaminase was achieved in Tris-HCl buffer at concentration of 0.15 M. Incubation of the… CONTINUE READING