Some kinetic properties of liver pyruvate kinase (type L). II. Effect of pH on its allosteric behavior.


Type L pyruvate kinase from rat liver has been further studied. The allosteric properties of the enzyme are strongly affected by changes in the hydrogen ion concentration of the assay medium. At pH values lower than 7, the enzyme obeys Michaelis-Menten kinetics with respect to one of the substrates (phosphoenolpyruvate (PEP)) and cannot be activated by… (More)


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